Taking Guilin City as a case study, an analysis has been made of the spatial distribution characteristics of rural tourism destinations in this city by using ArcGIS10.2, followed by an inquiry into its inherent distribution pattern, thus further proposing the development direction of rural tourism agglomeration in Guilin City. The research results indicate that rural tourism destinations in Guilin City is characterized with a significant feature of clustering distribution, with the jurisdiction of Guilin City and Lingchuan County being hotspots of rural tourism destinations, forming two core rural tourism areas in the central and southern regions. The rural tourism areas in Guilin City can be divided into three main clusters, namely the Red Rural Tourism Cluster, the Ecological Rural Tourism Cluster and the Ethnic Rural Tourism Cluster. Based on the spatial status of rural tourism destinations, it is therefore advisable for a wise utilization of a unique rural resources on the basis of existing advantages, so as to develop characteristic rural tourism industries, narrow the urban-rural gap, promot urban-rural integration, thus promoting the construction of Guilin as a world-class tourist city.