The ability of sensing and transmitting extracellular signals exhibited by somatic embryo genesis receptor-like kinases (SERKs) plays an important role in plant physiological processes such as growth and development. Base on the transcriptome data of Lilium oriental hybrids‘Siberia’, two SERK genes of Lilium, LoSERK1 and LoSERK2, have thus been coloned. The open reading frame of LoSERK1 has a total length of 1875 bp, encoding 624 amino acids and containing 53 potential phosphorylation sites, while the open reading frame of LoSERK2 has a total length of 1 887 bp, encoding 628 amino acids and containing 55 potential phosphorylation sites. The amino acid sequence similarity exceeds 90% between LoSERKs and plant SERKs such as Arabidopsis thaliana, Oryza sativa and Camellia sinensis. The phylogenetic tree analysis shows that the LoSERKs of Lilium are in the same group as the AtSERK1 and AtSERK2 of Arabidopsis thaliana, and two OsSERKs of Camellia sinensis. A real time quantitative PCR analysis shows that LoSERK1 is characterzied with a high relative expression level in both petals and anthers, while the relative expression level of LoSERK2 is high in open petals. It is speculated that LoSERKs are involved in the regulation of pollen development and floral organ abscission.