The comprehensive evaluation and protection of the ecological environment plays an important role in the development and construction of tourist villages. Taking Wolong Village of Chaling County as an example, with a comprehensive consideration given to the natural ecology and human social factors of the targeted area, six ecological factors reflecting the tourist villages characteristics has thus been selected according to the local conditions, including vegetation coverage, land use typed, flowing waters, biodiversity, slope direction and inclination, to construct the ecological sensitivity evaluation index system of Wolong Village. Based on GIS and RS, the multi-factor weighted superposition method is used for a comprehensive evaluation. The evaluation results show that the village area of Wolong Village is characterized with a high ecological sensitivity, of which the extremely and relatively highly sensitive areas account for 47.21% of the total area, with very few insensitive areas and low sensitive areas accounting for 20.15% of the total area. Taking into account the results of ecological sensitivity evaluation and the needs of tourist village development and construction, a proposal has been made of a“zoning construction” strategy, thus laying a foundation for scientifically delineating ecological protection and development areas and ensuring ecological security in villages, so as to realize a sustainable development of Wolong Village.