屠宰废水中COD降解菌分离鉴定与特性研究
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国家自然科学基金资助项目(51774128,31501538),湖南省科技厅重点研发基金资助项目(2016NK2096),长 沙市科技局重点基金资助项目(kh1601106),国家大学生研究性学习和创新性实验计划基金资助项目(201511535003)


A Research on the Isolation Identification of COD Degrading Strain with Its Characteristics in the Slaughterhouse Wastewater
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    摘要:

    通过选择性富集培养、驯化培养、划线分离和纯化等方法,从湖南某肉制品有限公司的屠宰废水中筛选到4株COD降解菌株,编号为1~4号。并对4株COD降解菌进行驯化与初步分离,采用高锰酸钾法测定各菌株的COD降解率,其中3号菌株的COD降解率最高,达到11.30%。经过观察3号菌株的形态特征,以及革兰氏染色实验,初步鉴定3号菌株为盐水球菌属细菌。并对其培养温度、pH值、蛋白胨浓度和酵母膏浓度等特性进行了研究,单因子优化实验结果表明,3号菌的最适生长温度为37 ℃、最适pH值为6、培养基中蛋白胨的质量分数为1.5%、酵母膏的质量分数为0.8%。

    Abstract:

    By means of selective enrichment cultivation, domestication, streaking cultivation and purification, four COD degrading strains, numbered 1, 2, 3 and 4 respectively, have been isolated and separated from slaughterhouse wastewater in a meat product Co., Ltd., Hunan Province. A preliminary separation has been made of the four domesticated strains of COD-degrading bacteria, and the potassium permanganate method has been adopted to determine the COD degradation rate of strains, with three of them reaching the highest rate of 11.30%. After observing the morphological characteristics of No. 3 strain and Gram staining test, it has been preliminarily identified that No. 3 strain proves to be Salinococcus. A research has been conducted on the characteristics of culture temperature, pH value, peptone concentration and yeast extract concentration, with the results of single factor optimization showing that the optimum growth temperature of No. 3 strain will be 37 ℃, with the optimum pH value 6, the concentration of peptone in culture medium 1.5%, and the yeast extract concentration 0.8%.

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贾明玺,李 文,邓 靖,刘 存,王莎莎,岑 烽,幸 芬,裴 鑫.屠宰废水中COD降解菌分离鉴定与特性研究[J].湖南工业大学学报,2019,33(1):60-65.

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  • 收稿日期:2018-04-12
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  • 在线发布日期: 2019-01-25
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