An ecological space network topology model can be extracted from the current data by using Pajek software, followed by an importance evaluation of the comprehensive key nodes. On the basis of the evaluation results, the identification of the ecological space network key nodes can be achieved, with a comparison made between the recognition results and the documented results, with the key nodes having greater centrality in the network structure, as well as a consistent location in the ecologically-sourced areas of high ecosystem service value. Researches on the identification of key nodes in the ecological network play a positive role in the process of improving the pertinence of the key areas of ecological planning, maintaining the stability of the ecological network and ensuring the operation of ecological functions.