The proanthocyanidins content was determined in rice grain of different colors using dimethylamino-cinnamaldehyde (DMACA) staining, Ferric ammonium alum staining and Vanillin staining. Experimental studies showed that the proanthocyanidins mass fraction of five rice breeds (Tannong s/zhi21, R288, Fan-14, 12Geng351 and Qingdao2) detected by DMACA staining was successively as follows, 0.136, 0.183, 0.085, 4.115 and 24.736 mg/g (dry weight); the result detected by Ferric ammonium alum staining was 0.292, 0.094, 1.940×10-4, 1.220 and 8.080 mg/g; and the result detected by Vanillin-HCl staining was 1.349, 1.363, 1.093, 3.733 and 15.153 mg/g. The proanthocyanidins content of Qingdao2 was the highest, which was about 4.1 to 6.6 times of 12Geng351, and Fan-14 was the lowest in five rice breeds; the proanthocyanidins content of black rice breeds (Qingdao2 and 12Geng351) was higher than that of white rice breeds (Tannong s/zhi21, R288, Fan-14). The relative standard deviation (RSD) of the absorbency showed that the precision level of determining proanthocyanidins content as follow, the first was vanillin staining, the second was DMACA staining and the third was ferric ammonium alum staining.