Used PCR-CTPP to detect the p73 gene polymorphism among 100 patients with NSCLC and 120 healthy controls respectively and DNA direct sequencing verified the results of genotypes. Analyzed the association between p73 gene polymorphism and NSCLC, and explored the correlation between p73 gene polymorphism and susceptibility to NSCLC in the Hunan population. The experiment results showed that the results of PCR-CTPP and the sequencing genotyping results were consistent, p73 genotype distribution in 100 lung cancer patients are as follows: GC/GC 60 cases (60%), GC/AT 36 cases (36%), AT/AT 4 cases (4%). Compared with the carriers of GC/GC genotype, the carriers of AT/AT genotype significantly increased 3.4 times the risk of NSCLC (OR=3.387, 95%CI= 1.055~10.875, P=0.032), also the carriers of GC/AT genotype tended to increase the risk of NSCLC, but had no significantly difference (OR=1.183, 95%CI= 0.672~2.082, P=0.561). The p73 gene polymorphism may have the relation with the susceptibility to NSCLC in the Hunan population.