Investigated the effect of calcium ions in SAS on the Zeta potential of quartz surface and the flotation process by means of flotation experiment, Zeta potential measurement, infrared spectroscopy analysis and calcium ion solution chemical calculation. The results show that Ca(OH)+ is the main factor to affect the Zeta potential of quartz surface, and Ca(OH)+ is the main active ingredient that activate flotation of quartz in the system of anionic collector dodecyl sulphate.