To investigate the urine indicators variation of college medium distance running athletes in the pre-competition training and provide a reference to the track and field training, selects 6 college athletes as objects of research and makes continuous tracking tests on the 10 indicators of the morning urine and after-sporting urine in 10 weeks before competition. Results show that the urine protein is the most sensitive indicator, ketone body-positive rate appears high after high intensity training and the next morning urobilinogen amount of some athletes is higher than that after the previous training. Therefore the urine composition analysis may reflect the athletes' functional status and can be used to judge the fatigue degree of athletes. And athletes' diet before match does not meet the demand of sports training, some shows unfit state in the pre-competition training and needs to adjust the training program.