To evaluate the food safety and provide a scientific basis for comprehensive development and utilization of hemerocallis fulva, the contents of microelements and heavy metals in hemerocallis fulva were measured by atomic absorption spectro-photometer after wave digestion. The results show that hemerocallis fulva is rich in minerals and trace elements. With arsenic as the research object，the toxicological safety evaluation tests were conducted，including acute oral toxicity test, rats bone marrow micronucleus test，rats sperm abnormality test and 180 d feeding test. The results show that water-soluble materials of arsenic, mercury, cadmium levels in the samples of 45 batches hemerocallis fulva are: w ( As) < 0.618 μg /g, w (Hg ) < 0.183μg/g and w (Cd ) ≤0.165 μg/g. The genetic toxicity test showed no significant correlation, 180 d feeding test showed that there was non-toxic and no side effects in the experimental dose range. The experiments indicate that the contents of microelements in hemerocallis fulva are rich and it has good food safety.