Aiming at the substrate depletion method widely used in multiple drug filtering, establishes a linear equation of enzymatic reaction based on drug decay curve and proposes a new method for linear conversion solution of enzymes kinetic parameters. Determines decay curves of nine probe metabolic reactions in human liver microsomes and obtains by linear regression the Michaelis constant Km, maximum velocity of the metabolic reaction Vmax and intrinsic clearance CLint. Comparing with traditional substrate depletion curvilinear fitting method, verifies the effectiveness of the new method. Multi-linear decay curve mapping is a simple, intuitive and reliable data visualization method.