Explicit and implicit stereotype of urban and rural were used to examine college students from Wuhan. Fifty six students participated in this study and they described main impression of the people from city and rural. Among these people, they picked up twenty attribute words and twelve conception words for city and rural people. The IAT were carried on for seventy two students among four hundred and sixty six college students from four universities after two weeks. The result showed that marked difference on impression between city people and rural people still existed. No significant correlation was found between explicit and implicit stereotypes of urban and rural stereotypes, as well as the function of subject gender and matriculate quality.