Polyacrylamide/Fe3O4 (PAM/Fe3O4) magnetically induced oriented hydrogels were successfully prepared by using acrylamide (AM) as the matrix material, N,N’-methylenebisacrylamide (MBAA) as the crosslinking agent and ammonium persulfate (APS) as the initiator under different magnetic induction conditions. The results of Fourier transform infrared spectroscopy showed that obvious intermolecular hydrogen bonds were formed between Fe3O4 and polyacrylamide. The mechanical properties and rheological properties of PAM/Fe3O4 magnetically induced oriented hydrogels were analyzed by strain-controlled rotational rheometer, and the orientation structure of the hydrogels was analyzed by metallographic microscope. The results showed that the tensile strength and elongation at break of the parallel magnetic field oriented hydrogel were better than those of the vertical magnetic field oriented hydrogel. The mechanical properties of parallel magnetic field oriented hydrogels were better than those of zero magnetic field oriented hydrogels. With the increase of magnetic induction intensity, the tensile strength of PAM/Fe3O4 magnetic induction oriented hydrogel became larger, and the orientation structure was more obvious. With the increase of AM content, the mechanical properties of hydrogels increased first and then decreased. The PAM/Fe3O4 magnetically induced oriented hydrogel has a good application prospect in the field of magnetic response intelligent bionic materials.