Highly transparent pressure-sensitive adhesives are widely used in display screens, cell phones, automobiles and other fields, but they generally have poor yellowing resistance and low bonding performance in practical applications and high-temperature conditions. Taking 2-Ethylhexyl acrylate (2-EHA) and butyl acrylate (BA) as soft monomers, isobornyl acrylate (IBOA) as hard monomers, hydroxyethyl acrylate (2-HEA), glycidyl methacrylate (GMA), acrylic acid (AA) as functional monomers, and (2, 4, 6-trimethylbenzoyl) diphenylphosphine oxide (TPO) as the photoinitiator, the prepolymer was prepared by bulk polymerization initiated by UV light. Then a highly transparent, cooling-heating cycling resistant UV-cured pressure-sensitive adhesive was prepared by using 1,6-hexanediol diacrylate (HDDA) as the reactive diluent, TPO and 2-hydroxy-2-methyl-1-phenyl-1-propanone (1173) as the photoinitiator, with the addition of antioxidant 1010. Characterization methods such as rheometer, thermogravimetric analyzer and UV spectrophotometer were used to investigate the effects of the formulation design of the pressure-sensitive adhesive on its adhesive properties, cooling-heating cycling resistance and yellowing resistance. The results show the initial adhesion of the pressure-sensitive adhesive for the No. 10 steel ball, 180 ° peel strength of 25 N/25 mm, holding time greater than 100 h, which can effectively solve the issues of low bonding performance in pressure-sensitive adhesive for display screens, being prone to foaming, shrinkage and poor yellowing resistance at high temperatures.