Aiming at the problem of poor mechanical properties of traditional fluorescent hydrogels, based on the nano-toughening strategy, with Agar as the first network, polyacrylamide (PAAM) as the second network, and NH2-MIL-53(Al) as the nano-toughening agent with fluorescence characteristics, NH2-MIL-53(Al)/Agar/PAAM high strength nanocomposite fluorescent hydrogels were prepared by heating-cooling-photopolymerization. The structure and particle size of NH2-MIL-53(Al) were characterized by X-ray diffraction，Fourier transform infrared spectroscopy and dynamic light scattering, the mechanical properties of the hydrogel were tested by a universal testing machine, and the fluorescence properties of the hydrogel were tested by a fluorescence spectrophotometer. The results show that the gel has high mechanical strength (tensile stress was 1.0 MPa, tensile strain was 960%), and exhibits strong fluorescence properties under 450 nm photoexcitation. The NH2-MIL-53(Al)/Agar/PAAM nanocomposite fluorescent hydrogels provide a new idea for the preparation of fluorescent hydrogels with high mechanical properties and promote the application and development of fluorescent hydrogels.