Hydrogen sulfide (H2S) plays an important regulatory role in organisms. However, it is highly toxic, and once the concentration exceeds physiological levels, it can damage the respiratory system, nervous system and even heart organs. A H2S-sensitive fluorescent probe DHBID based on 1, 8-naphthimide was designed and synthesized. Experimental results showed that fluorescence intensity at 480 nm was significantly enhanced with the increase in Na2S concentration, with the signal-to-background ratio up to 15 times. With the Na2S used as H2S donor, the probe DHBID showed an obvious response signal to H2S in the range of 0~250 nmol/L, and had a good linear relationship in the concentration range of 5~100 nmol/L with its correlation equation expressed as Y = 19.258X +319.460 (R2 = 0.9758), with the detection limit of 2 nmol/L. The probe was highly sensitive to H2S with a relatively short reaction time, and highly selective with no response to common anions. Besides, within response range, with the increase in H2S concentration, the color of the reaction solution gradually changed from light yellow to rose red, which could realize the visual detection of H2S with different concentrations.