Short-chain amylose was prepared by debranching waxy starch with pullulanase. The starch film was dipped in the short-chain amylose solution gelatinized by microwave, and then aged at 4℃ to get superhydrophilic starch films. The surface morphology, crystal structure, infrared spectrum and hydrophilicity of superhydrophilic starch film were studied. The experimental results showed that nanoparticle structure of short-chain amylose was self-assembled on the surface of starch film, which increased the surface roughness and improved the hydrophilicity of starch film. When the mass concentration of short-chain amylose solution was 0.010 g/mL, the superhydrophilic starch film with a water contact angle close to 0° could be obtained.