Nitrogen doped graphene oxides (NGOs) were prepared by hydrothermal method with adenine as nitrogen source, trimesic acid as auxiliary carbon resource, and through calcinating precursors in an inert atmosphere. The dosage effects of adenine were deeply studied. The catalytic performance for oxygen reduction reaction （ORR）of as-prepared catalysts was analyzed by linear sweep voltammetry on the electrochemical workstation CHI760E (ChenHua, ShangHai), and the electron transfer numbers of oxygen reduction reactions were calculated by Koutecky-Levich equation. As results, the oxygen reduction catalytic performance of NGOs was greatly improved with different usages of adenine. When adenine was 6mmol, the catalytic performance was the best with catalyzed oxygen reduction through a 4e-1 pathway.