The shelf life model of fish balls packed at room temperature was studied for the convenience of online shops selling fish balls to control the shelf life more accurately. According to the TVB-N value of fish balls, a shelf life model based on reaction kinetics and a two element nonlinear shelf life model with the help of statistical tools (1stOpt and MATLAB) were established. Then, the accuracy of the two shelf-life models was tested by the total numbers of colony. The results showed the primary reaction kinetics shelf life model had to be calculated by the conversion of a large number of data and formulas, but it could better explain the relationship between the temperature and the shelf life in spite of the complex process. The two element nonlinear shelf life model was simple and easy to calculate with the help of statistical tools, but it could not explain the relationship between the temperature and the shelf life. Moreover, the calculation of shelf life had to resort to professional software of MATLAB, which made it hard in application for common online shops. Both of the two models demonstrated certain degree of accuracy, the primary reaction kinetics shelf life model was recommended for common users since it was more precise.