TiO2/electrochemically reduced graphene composite modified glassy carbon electrode (TiO2-ErGO/GCE) for the purpose of sensitive detection of Tartrazine was prepared by dropping-casting followed by an electrochemical reduction method. The prepared material was characterized by transmission electron microscopy (TEM) and X-ray diffraction (XRD). Cyclic voltammetry and second-order derivative linear scan voltammetry were performed for analyzing the electrochemical sensing of tartrazine on different electrodes. The determination conditions (including pH value, accumulation potential as well as time) were optimized systematically. The results showed that the TiO2-ErGO composites increased the electrochemical active area and enhanced the electrochemical responses of tartrazine significantly. The optimal pH was 3.7. The optimal accumulation potential and time were -0.2 V and 180 s respectively. Under the optimum detection conditions, the peak current was found to be linear with tartrazine concentration in the range of 2.0×10-8~2.0×10-5 mol/L with a lower detection limit of 8.0×10-9 mol/L (S/N:3). The proposed TiO2-ErGO/GCEs have been successfully applied in the detection of tartrazine in soft drink samples with satisfactory results.