Ultraviolet Curable Polyurethane Acrylate (PUA) was prepared with two-step method by using Poly(ethylene glycol)-400 (PEG-400) as the soft segment，with Ethylene glycol monoacrylate (HEA) and Toluene-2,4- diisocyanate (TDI) as the hard segment. FTIR and H-NMR test result indicated the successful preparation of PUA. The synthesis conditions and mechanical properties were studied. The experimental results indicated that the optimum synthetic condition in the first step was 75℃, 3 h, while 45℃, 5 h was the best condition for the second step. The preferred mass fraction of the polymerization inhibitor was 0.5%. 1,6-Hexanediol diacrylate (HDDA) and Trimethylolpropane triacrylate(TMPTA) with a mass ratio of 1:1 being added into the PUA. Under this condition, the cured films had the highest gel fraction and excellent overall performance. In addition, using the compound photoinitiator system made the coating to be of the highest curing rate and the optimal mass fraction was 3%.