Antioxidant extracted from bamboo leaf was added into hydroxypropyl methyl cellulose(HPMC) solution to prepare packaging films by casting method. The transformation of chemical structure and crystallization conditions of the film were characterized by FT-IR, X-ray and TG. The transmittance and haze of the sample film were measured, while oxidation resistance properties of the film with different AOB contents were analyzed by the experiment of scavenging DPPH radicals. The performance of the film including anti-oxidization, water-solubility, mechanical properties and optical properties with different contents of AOB additive were studied. The results showed that the addition of AOB has not destroyed the chemical group of HPMC while only disturbed the arrangement of the molecular chains. The crystallinity of the AOB/HPMC composite film decreased, with the increase in temperature of thermal decomposition and the enhancement of thermal stability, which indicated the anti-oxidation properties. Meanwhile, the water solubility of composite water-soluble packaging film increased with the increasing amount of AOB, with the slight decrease in mechanical properties and optical properties, while the antioxidation increased firstly and then decreased. When the addition amount of AOB was 0.03wt%, the scavenging rate of DPPH free radicals reached a maximum value of 89.34%.