Taking E.coli and Aspergillus niger as examples, antibacterial and antifungal effects of silver-loaded zeolite with different additions in kraft paper were detected. Result shows that when the concentration of antibacterial agent in paper is 1 000 mg/L, the inhibition zone reaches 21.5 mm. It can be seen that the growth of E.coli can be inhibited greatly by silver-loaded zeolite. While the quality of antibacterial agent added in paper is 1.0 g, Aspergillus niger can be inhibited effectively in paper and mycelium cannot grow at the edge of paper, so silver-loaded zeolite has a certain effect on Aspergillus niger. Silver-loaded zeolite with different additions have has very limited impact on mechanism property, whiteness, smoothness of paper. Therefore, While the additions of antibacterial agent are between 0.5 g and 1.0 g, the growth of E.coli and Aspergillus niger can be effectively inhibited by antibacterial paper while mechanism properties, whiteness, smoothness of these papers are not dramatically affected.