Fluorescence quantum yield is one of the most important parameters of fluorescent materials. The fluorescence excitation spectra and fluorescence spectra have been tested, and the fluorescence quantum yields of fluorescent inkjet ink samples under different excitation wavelength have been calculated with relative measurement method. The effect of solvent types and the content of resins on the fluorescence quantum yield have been measured. The results indicate that the polarity of the solvents and the content of resins have important influence on the fluorescence quantum yield. The fluorescence quantum yield reached the maximum value 0.96 when the dipropylene glycol monomethyl ether was used as the solvent, and compared with 8%, the fluorescence quantum yield was higher when the content of resin was 10%.