激发-发射矩阵荧光光谱用于鉴别百合掺假的研究
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湖南省教育厅科学研究基金资助优秀青年项目(22B0579)


Study on Authenticity Evaluation of Adulteration in Lily Using Excitation-Emission Matrix Fluorescence Spectroscopy
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    摘要:

    为了能够快速判别百合是否掺假,利用激发-发射矩阵(EEM)荧光技术对纯百合和掺假百合样品进行了荧光光谱分析,并构建了百合及其掺假百合的荧光指纹特征图谱;然后借助主成分分析-线性判别分析(PCA-LDA)和偏最小二乘-判别分析(PLS-DA)两种化学模式识别方法,对百合中掺假粉末的种类进行了快速鉴别和分类。实验结果表明:两个分类模型均能根据百合样本的EEM荧光光谱数据准确识别掺假百合样本,且正确分类率均高达95%。利用PCA-LDA和PLS-DA成功建立了快速判别百合掺假的新方法,同时完善了百合荧光指纹特征图谱,有望为建立更全面、更准确地评价百合药材的质量标准体系打下基础。

    Abstract:

    In order to quickly identify the adulteration in lily, pure lily and adulterated lily were analyzed by excitation-emission matrix (EEM) fluorescence spectroscopy technique, and the fluorescence fingerprints of lily and adulterated lily were constructed. Then, two chemical pattern recognition methods, namely principal component analysis linear discriminant analysis (PCA-LDA) and partial least squares discriminant analysis (PLS-DA), were used to identify and classify the adulterated powder of lily. The experimental results show that both two classification models can accurately identify adulterated lily samples using EEM fluorescence spectrum data, and the correct classification rates (CCRs) of both models are above 95%. Using PCA-LDA and PLS-DA, new methods for rapid identification of lily adulteration were successfully established, and the fluorescence fingerprint of lily was improved, which is expected to lay a foundation for establishing a more comprehensive and accurate quality standard system for evaluating lily.

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唐 英,张思维,张芷柔,陈 瑶.激发-发射矩阵荧光光谱用于鉴别百合掺假的研究[J].包装学报,2023,15(6):62-72.

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  • 收稿日期:2023-09-10
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  • 在线发布日期: 2024-01-05
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