A pair of specific monoclonal antibodies were prepared and screened by using tissue type plasminogen activator inhibitor complex (t-PAIC) as immunogen, and then a magnetic particle chemiluminescence immunoassay method was established for the detection of human plasma t-PAIC. In comparison with the reference method, the one-step method with a faster reaction speed was finally selected as the reaction mode. Then the performance of the method was estimated, the results showed that the limit of blank (LOB) was 0.43 ng/mL, the limit of detection (LOD) was 0.91 ng/mL, and the linear range was 0.91~100 ng/mL. The coefficient of variance (CV) of repeatability was less than 4%, and the CV of precision was less than 3%. The sample recovery rate was between 100%~110%, and the common interfering substances had no obvious influence on the detection results. Finally, the reference interval of the method was constructed. The reference range for healthy adult males was 1.85~15.91 ng/mL, and that of 1.90~9.43 ng/mL for healthy adult women. The method has the advantage of rapid reaction, which is of important application value in the diagnosis of thrombotic diseases.