Rapid early diagnosis of TORCH infection is crucial in disease control and helps to improve the effectiveness of diagnosis and treatment, and reduce medical risks. An immunoassay for simultaneous detection of IgG specific antibodies against Toxoplasma gondii, rubella virus, cytomegalovirus and herpes simplex virus was established using multiplex fluorescence coding technique. The optimal assay conditions were achieved by optimizing the pH of the coupling buffer and the concentration of Goat anti-Human IgG-PE. The performance of the modified experimental method was verified in terms of precision, specificity, dose-response curve and detection limit. The results showed that the relative standard deviation (RSD) of each item was less than 10%, and there was no cross-reactivity with antibodies to a variety of common pathogens. The batch testing of five items could be completed simultaneously within 40 minutes, and the results were well correlated with those tested by CLIA method. This method can shorten the test cycle and reduce testing cost, providing a new option for rapid, sensitive and high-throughput TORCH infection screening in the clinic.