Abstract:Lipase microcapsules were prepared by adopting ion gel technology in order to solve the problems of poor thermal stability performance and great influence of temperature and pH value on lipase activity in the application of time-temperature indicator. The preparation process of lipase microcapsules was optimized by single factor test and orthogonal test with the concentration of sodium alginate, chitosan and calcium chloride as variables with the thermal stability, optimum temperature and pH value of lipase and lipase microcapsules were studied. The structure and morphology of lipase microcapsules was analyzed by infrared spectroscopy (FTIR) and scanning electron microscopy (SEM), and then the application of lipase microcapsule in time-temperature indicator was preliminarily studied. The optimal preparation conditions of lipase microcapsules were as follows: sodium alginate mass concentration was 30 g/L, chitosan mass concentration was 6 g/L, calcium chloride concentration was 0.3 mol/L, and the thermal stability of lipase microcapsules was significantly improved compared with the original lipase. The preparation technology of lipase microcapsule is stable and reliable, which has practical value in time-temperature indicator.