An integrated, rapid and simple immune microfluidic chip was developed by using microfluidic chip technology to detect four respiratory virus antigens visually through colloidal gold colorimetric detection. The results showed that the lowest detection limits for influenza A antigen, influenza B antigen, RSV antigen and severe acute respiratory syndrome coronavirus antigen were 37.6 ng/mL, 17.8 μg/mL, 112.5 ng/mL and 0.05 ng/mL, respectively, and the detection time was 10 min, indicating good specificity. This method can realize the rapid detection of respiratory viral antigens on site and distinguish COVID-19 from common respiratory infections in a timely manner, which can improve the efficiency of epidemic prevention and control.