Taking Agaricus bisporus as the raw material, the effects of 1 h and 2 h vibration treatments (with unvibrated mushrooms as a control) on the color, active oxygen metabolism and antioxidant properties of fresh Agaricus bisporus under 3 Hz vibration frequency were studied. The results showed that vibration stress accelerated the production of O2- and the accumulation of H2O2 content, the O2- production of the Agaricus bisporus treated with different vibration time was 2.89 times (1 h) and 3.31 times (2 h) of the control group, H2O2 content was 1.38 times (1 h) and 1.56 times (2 h) of the control group. After vibration stress, SOD, CAT and APX antioxidant enzyme activities, relative conductivity and MDA content increased significantly. In addition, ascorbic acid decomposition accelerated, total phenols, flavonoids content and PPO enzyme activity peaks appeared earlier. Due to the large accumulation of active oxygen free radicals, the oxidative damage of the cell membrane was aggravated and eventually accelerated the browning of the mushrooms.