Abstract:Aiming at the issue of the limited use of plant fibers in paper making and control treatment industries due to their length and tenacity, the screening of fiber degumming bacteria RJ6 was isolated and screened from the rotting vegetable, and the strain was inoculated into a medium with ramie juice as the only organic nutrient component to perform degumming experiments on ramie. Morphological identification, physiological and biochemical characteristics and 16S rDNA sequence comparison were carried out, and the fermentation conditions of the strain were optimized. The results showed that the degumming rate of the experimental group inoculated with RJ6 was 91%, compared to the control group, indicating that RJ6 had an efficient degumming effect on plant fiber raw materials. By optimizing the fermentation conditions of strain RJ6, it was shown that the RJ6 maintained a high level of fermentation under the conditions of 160 r/min, 5% inoculation, 37℃ and initial pH 7. The results of morphological identification, physiological and biochemical characteristics experiment, and 16S rDNA sequence alignment showed that RJ6 was short rod shaped, blunt round at both ends, approximately (0.4~0.5) μm×(1.1~1.8) μm in size. The test results were negative for Gram staining, and there were terminal flagella. The 16S rDNA sequence of RJ6 was more than 99% homology with Pectobacterium.sp from GenBank, and it also showed that RJ6 belonged to Pectobacterium.sp.