The novel Ta2O5/Ta2O5-Ti/Ti multilayer coatings were prepared on the surface of Ti6Al4V alloy by magnetron sputtering. The morphology, phase composition and elemental chemical state of the coating were investigated by scanning electron microscopy (SEM), X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS). The bonding strength, hardness, wear behaviour and corrosion resistance of the coating were respectively evaluated by scratch tester, friction and wear tester and electrochemical workstation. The results showed that the main features of the coating surfaces were characterized by peak-type particles with uniform size and compact structure. Ta2O5/Ta2O5-Ti/Ti multilayer coatings showed a lower friction coefficient, higher corrosion potential and smaller corrosion current density compared with Ti6Al4V alloy, indicating good tribological properties and corrosion resistance, which could provide a good protection for Ti6Al4V alloy implant material in orthopedic applications.