Polythiophene and its derivatives have the advantages in high electrical conductivity, environmental protection, and thermal stability. It can be prepared into conductive ink, which has great application prospects in the fields of printed electronics and smart packaging. PEDOT-CB/CMC conductive ink was prepared by in-situ polymerization. The effects of binders, auxiliary additives and sintering temperature on ink viscosity, adhesion, dispersibility and electrical conductivity were systematically studied. The printability of the ink was also studied by means of screen-printing technology. The results showed that the conductive ink had good dispersibility and adhesion properties. After the heat treatment of the printed product, the resistance value decreased significantly, while the resistance value would change regularly with the increase in the length and width of the printed product. In addition, the ink could meet the requirements of screen-printing on different substrates such as paper, plastic and cotton.