According to the specific gene (malB) of Escherichia coli (E.coli), the primers of loop mediated isothermal amplification (LAMP) were designed and screened, and the optimum LAMP reaction system was obtained by optimizing the reaction conditions such as betaine dosage, concentration of loop primer, reaction temperature and dNTPs dosage. Then the optimal method was established for rapid detection of E.coli by LAMP real-time turbidity method. In the optimal conditions, 9 strains of non-Escherichia coli strains were tested for specificity and 5 strains of Escherichia coli were tested for homology. Moreover, using the multichannel turbidity device developed by the research group, the produce of LAMP could be real-time monitored. The results showed that the sensitivity of E.coli was 16.010 ng/L, in line with the detection limit of real-time fluorescence quantitative PCR. Therefore, the optimization and establishment of real-time turbidity LAMP method could provide a powerful approach to rapid field-detection of E.coli in food.