The kinetics of immobilized glucoamylase time-teruperature indicators（TTIs）was studied, the activation energy and reaction endpoint of TTIs was achieved, with the research on yogurt activation energy and shelf life. According to the activation energy and ending point matching principle between TTIs and the perishable, the matching experiments were set up both at constant and variable temperature. As a result, 1# TTI was verified to predict the shelf life of yogurt more accurately with the activation energy matching yogurt and the errors of reaction endpoint within 15% based on experiments in yogurt quality monitoring .