More and more researchers applied inkjet printing technology into the fabrication of electrochemical biosensors because of its unique features, such as non-contact, simple process and no version digitization in order to deal with the higher requirements from the developments of electrochemical biosensors into digitization and intelligence. The principle and the advantages and disadvantages of the electrochemical biosensors were analyzed based on inkjet printing technology. According to its development process, the recent researches have been summarized and analyzed, with the existing problems in the process of production discussed, especially the issue of relatively high requirement of the ink. The focus in the research in future would be finding a suitable method to solve the problem of the conductivity of the ink jet printing metal electrode, studying the influence of the concentration of the conductive polymers and layers on film quality and sensor response, and using inkjet printing technology in the preparation of the whole sensor.