The objective evaluation method of printing image quality has not achieved good agreement with the subjective evaluation, and the reference image quality assessment method was too dependent on the reference image, which could not be used in real-time printing image quality detection system. A quality evaluation model NIQE which could do without a subjective evaluation score was proposed and applied in objective evaluation of the printed image quality to evaluate the overall quality with the combination of subjective experiments. NIQE algorithm’s results on a subjective evaluation of the correlation analysis showed the CC value being 0.910 1, the SROCC value 0.946 1, the MAE value 0.016 0, compared to the four full reference algorithms MSE, PSNR, SSIM and MS-SSIM and a no-reference evaluation algorithm BIQI, the NIQE algorithm performed better in stability, monotony and consistency. Therefore, NIQE algorithm subjective quality evaluation of the human eye had a better consistency, closer to the human visual system and could effectively evaluate the real time quality of the printed image.