A natural lighting and glare simulation test has been carried out on the public buildings in a university in Zhuzhou, Hunan Province by using a comprehensive building performance simulation software Ecotect Analysis and a luminous environment simulation software Desktop Radiance, thus working out the natural daylighting coefficient and glare index, followed by an analysis of the natural daylighting effect of the main classrooms in public teaching buildings of standard level. The results show that the natural lighting coefficient and glare index of the main classrooms in the public teaching buildings meet the requirements of the building lighting design standard (GB 50033—2013) and the green building evaluation standard (GB/T 50378—2014).