This paper studies a pricing problem of a two-channel supply chain consisting of a single manufacturer and a single e-commerce platform sellers. Considering the market where consumers are sensitive to the delivery time of fresh products, and from the perspective of decentralized decision making and centralized decision making, this paper analyzes the impact of delivery time of fresh products on the pricing and earnings of manufacturers, e-commerce platform proprietors. The results show that the wholesale price of the manufacturer is negatively correlated with the product delivery time of the proprietor of the e-commerce platform, and the pricing of products is also negatively related to the delivery time of products in their own channels. The product delivery time of the channel has spillover effect on the product price and profit of other channels. Manufacturers have more advantages in centralized decision making, whereas e-commerce platform operators are on the contrary.