In view of the increasingly stringent environmental requirements, a new type of eco-friendly glass cleanser has been developed. The optimal combination of formula has been obtained as follows: NaOH 2.0 wt%, SDS 0.3 wt%, FAMES 0.3 wt%, APG 0.3 wt%, ethanol 7.5 wt%, ethylene glycol 7.5 wt%, sodium citrate 0.2 wt%, triethanolamine 0.2 wt%, and EDTA disodium 0.5 wt%. The cleaning process has been optimized and repeated cleaning experiments have been carried out on this basis. The biodegradability of the glass cleanser has been investigated by measuring the COD content of the test solution based on shaking-bottle incubating tests. The results show that the cleaning efficiency proves to be the highest when the glass specimens are supersonically rinsed for 4 min at 25 ℃,with its removal rate being 99.46% and light transmittance being 88.05%. After seven times of of repeated cleaning, the removal rate is as high as 98.99%; and 28 days later, COD of the test solution content has decreased by 95.99%, thus verifying the good biodegradability of the new type of glass cleanser.