With the development of the non-destructive testing technology, Photoacoustic imaging technology has become one of the research focuses in biomedical engineering. With an annular array detector as imaging platforms and high-frequency pulse laser as a radiation source, the experiment adopted a filter back-projection algorithm to rebuild images and applied the photoacoustic tomography for fast imaging of rabbit brain. Experimental results showed that the system realized non-destructive, fast and efficient rabbit brain imaging, which revealed how to improve the imaging resolution of large size animals and further provided solid favorable technical conditions for medical nondestructive testing.