重铬酸钾对玉米根尖毒性效应的细胞遗传学研究
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Cytogenetic Toxic Effects of Potassium Dichromate on Root Tip Cells of Zea mays L.
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    摘要:

    以玉米(Zea mays L.)根尖为材料,研究了重铬酸钾(K2Cr2O7)对玉米根尖的致畸效应。以不同浓度的重铬酸钾为诱变剂,测定玉米根尖细胞的微核率、有丝分裂指数和染色体畸变率。结果表明:重铬酸钾能诱发较高频率的微核率,即在一定的质量浓度范围内(0~25.0 mg/L)其微核率随重铬酸钾处理浓度的升高而增加,但高于一定质量浓度(25.0~100.0 mg/L)反而呈下降趋势;不同质量浓度的重铬酸钾均能使玉米根尖细胞有丝分裂指数增大,还能诱导玉米根尖细胞产生较高频率的染色体畸变,在质量浓度为50.0 mg/L时,玉米根尖细胞的有丝分裂指数最大;畸变率最高,且产生多种类型的染色体畸变。因此,重铬酸钾对玉米根尖细胞具有明显的致畸效应,在一定范围内可以指示环境中重铬酸钾的污染程度。

    Abstract:

    The aberrant effects of different concentrations of potassium dichromate was studied by using Zea mays L. root tip cells. The micronucleus and chromosome aberration assay were conducted to determine the micronucleus frequencies and chromosome aberration frequencies of Zea mays L. root tip cells which induced by potassium dichromate. The result indicated that potassium dichromate could increase the micronucleus frequencies of Zea mays L. root tip cells. With certain range of concentration(0.0-25.0 mg/L) the frequencies of micronucleus was found to be increased with the increase of potassium dichromate concentration but beyond this range(25.0-100.0 mg/L) the frequencies of micronucleus decreased with further increase of potassium dichromate concentration. The potassium dichromate at different concentrations could increase the cell mitosis index. Besides, It also caused various types of chromosome aberration and the frequencies of chromosome aberration were always higher than that of the control group. The conclusion of this study was that potassium dichromate has obvious teratogenic effect on Zea mays L. root tip cells. In some degree the micronucleus frequencies of plant cells was able to show the grades of pollution of potassium dichromate in the environment.

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李国庆,赵 艳,易 岚,殷 杰,贺庆芝,秦志峰,刘运莲.重铬酸钾对玉米根尖毒性效应的细胞遗传学研究[J].湖南工业大学学报,2007,21(4):51-54.

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  • 收稿日期:2007-05-22
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  • 在线发布日期: 2015-09-02
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